Imaging Biomolecules Across Scales: From Atoms to Tissues (X6)
March 23-26, 2025
| Keystone Resort, Keystone, CO, United States
Elizabeth Villa, Bridget Carragher and Ilaria Testa
Scholarship Deadline: Jan. 24, 2025 | Abstract Deadline: Feb. 28, 2025 | Early Registration Deadline: Feb. 5, 2025
| 4:00–8:00 PM |
Registration |
Longs Peak Foyer |
| 6:00–8:00 PM |
Welcome Mixer |
Longs Peak Foyer |
| 7:00–8:00 AM |
Breakfast |
Shavano/Torreys Peaks |
| 8:00–9:00 AM |
Welcome and Keynote Address (Joint) |
Longs Peak/Grays Peak |
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* Elizabeth Villa, University of California, San Diego
Session Chair |
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* Mohammed AlQuraishi, Columbia University
Session Chair |
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Wolfgang P. Baumeister, Max Planck Institute of Biochemistry
Cryo-Electron Tomography or the Power of Seeing the Whole Picture |
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| 9:00–11:30 AM |
Machine Learning for Protein Structure (Joint) |
Longs Peak/Grays Peak |
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* Julia Rogers, Columbia University
Session Chair |
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Hannah Wayment-Steele, University of Wisconsin-Madison
Have Protein Language Models Learned Dynamics |
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Sergey Ovchinnikov, MIT
Hacking AlphaFold to Do New Things |
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* Mohammed AlQuraishi, Columbia University
Understanding How AlphaFold Learns |
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Fabian C Spoendlin, University of Oxford
Short Talk: Predicting the Conformational Flexibility of Antibody and T-cell Receptor CDRs |
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Katrina Black, UCSF
Short Talk: Improving Cryo-EM of Vesicle-Embedded Membrane Proteins: A Machine Learning Pipeline for Lipid Bilayer Subtraction |
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| 9:30–9:50 AM |
Coffee Break |
Longs Peak Foyer |
| 11:30–1:00 PM |
Poster Setup |
Shavano/Torreys Peaks |
| 11:30–2:30 PM |
On Own for Lunch |
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| 1:00–10:00 PM |
Poster Viewing |
Shavano/Torreys Peaks |
| 3:00–4:30 PM |
Symposia Spotlight 1: Orthogonal Technologies |
Longs Peak |
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* Bridget Carragher, Chan Zuckerberg Imaging Institute
Session Chair |
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Aneesh Deshmukh, University of Southern California
3D Cell Mapping of Insulin Secretory Granule Maturation using Soft X-ray Tomography |
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Margot Riggi, MPI for Biochemistry
Bringing it All Together: 3D Animation for Integrative Modeling of Complex Molecular Mechanisms |
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Christopher J Ackerson, Colorado State University
Cloneable Inorganic Nanoparticles |
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Yujia Zhang, Oregon Health Science University
Multiplexed Super-resolution Imaging of Cells and Clinical Tissue Sections from Nanometer to Millimeter Scales |
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Zhuowen (Kevin) Zhao, Chan Zuckerberg Imaging Institute
The Machine Learning Challenge for CryoET Annotation: A report on the Winning Models |
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Josh Hutchings, Chan Zuckerberg Imaging Institute
Structural Analysis of Lysosomal Proteins via Cryo-Electron Tomography of Affinity-Captured Organelles |
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| 4:30–5:00 PM |
Coffee Available |
Longs Peak Foyer |
| 5:00–6:45 PM |
Structure Determination of Challenging Complexes in vitro and in situ |
Longs Peak |
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Lori A Passmore, MRC Laboratory of Molecular Biology
Imaging DNA Repair Proteins, One Molecule at a Time |
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Dimitry Tegunov, Genentech
Enabling High-Resolution Structure Determination In Situ |
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Eshan Mehrotra, University of California, San Francisco
Short Talk: Cryo-ET Reveals Novel Assemblies in Olfactory Cilia |
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Sheena Vasquez, Stanford University
Short Talk: Towards Structurally Characterizing Nitrosomonas Europaea Enzymes in vivo for Wastewater Nitrogen Refinement |
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* Andres E Leschziner, UC San Diego
Short Talk: Regulation of LRRK2: Identifying Vulnerabilities for Parkinson’s Disease Therapeutics |
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| 7:00–8:00 PM |
Social Hour with Lite Bites |
Shavano/Torreys Peaks |
| 7:05–8:00 PM |
Women in Science Networking Session |
Crestone Foyer |
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Elizabeth Kellogg, St. Jude Children's Research Hospital
Session Chair |
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Elizabeth Villa, University of California, San Diego
Session Chair |
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Bridget Carragher, Chan Zuckerberg Imaging Institute
Session Chair |
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Suliana Manley, École Polytechnique Fédérale de Lausanne
Session Chair |
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| 7:30–10:00 PM |
Poster Session 1 |
Shavano/Torreys Peaks |
| 7:00–8:00 AM |
Breakfast |
Shavano/Torreys Peaks |
| 8:00–11:00 AM |
CryoET/EM and Machine Learning (Joint) |
Longs Peak/Grays Peak |
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* Allison Doerr, Springer Nature
Session Chair |
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Bridget Carragher, Chan Zuckerberg Imaging Institute
Tools and Technologies for Accelerating cryoET |
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* Bronwyn A Lucas, University of California Berkeley
Single Molecule Localization and Structure Probing in Cells |
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Elizabeth Villa, University of California, San Diego
Discovering New Biology with Cryo-ET |
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Elizabeth Kellogg, St. Jude Children's Research Hospital
Structural Insights into CRISPR-Based Genome Editing |
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Yuriy Chaban, Diamond Light Source ltd.
Short Talk: Automated Filtering of Particle Images in Single Particle CryoEM |
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| 9:00–9:20 AM |
Coffee Break |
Longs Peak Foyer |
| 9:20–9:25 AM |
Award Recipient Acknowledgement |
Longs Peak/Grays Peak |
| 11:00–1:00 PM |
Poster Setup |
Shavano/Torreys Peaks |
| 11:00–5:00 PM |
On Own for Lunch |
|
| 1:00–10:00 PM |
Poster Viewing |
Shavano/Torreys Peaks |
| 3:00–4:30 PM |
Career Roundtable (Joint) |
Grays Peak |
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Kate L. White, University of Southern California
Assistant Professor |
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Christina Y Ivashchenko, Novo Nordisk
Director of Scientific Projects |
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John E Mahoney, Cystic Fibrosis Foundation
Head of Stem Cell Biology |
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Ernesto Andrianantoandro, Cell Press
Scientific Editor, Cell Systems |
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| 4:30–5:00 PM |
Coffee Available |
Longs Peak Foyer |
| 5:00–6:45 PM |
The Big Picture: Capturing Events with Large-Volume EM and CLEM |
Longs Peak |
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Virginie Hamel, University of Geneva
Unveiling the Architecture of the Cell by Combining cryoET and Ultrastructure Expansion Microscopy |
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Adam Frost, Altos Labs
Predictions and Probes of Membrane Structure |
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* Kate L. White, University of Southern California
Soft X-Ray Tomography: Unraveling the Mysteries of Cellular Biochemistry |
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Ami U Thakrar, Massachusetts Institute of Technology
Short Talk: Time-resolved High-pressure Freezing for Dynamic, Nanoscale Imaging |
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| 7:00–8:00 PM |
Social Hour with Lite Bites |
Shavano/Torreys Peaks |
| 7:30–10:00 PM |
Poster Session 2 |
Shavano/Torreys Peaks |
Wednesday, March 26, 2025
| 7:00–8:00 AM |
Breakfast |
Shavano/Torreys Peaks |
| 8:00–11:15 AM |
Light Microscopy Comes to Structure |
Longs Peak |
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Eric Mulhall, The Scripps Research Institute
Linking the Structure, Mechanics, and Function of PIEZO Ion Channels with Fluorescence Nanoscopy |
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* Ilaria Testa, KTH Royal Institute of Technology
From Cells to STARSS: Monitoring the Life of Proteins at the Nanoscale |
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Joerg Bewersdorf, Yale University
All-Optical Super-Resolution Imaging of Molecules in Their Nanoscale Cellular Context |
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Francisco Balzarotti, Research Institute for Molecular Pathology
Accessing the Nanoscale with Light Microscopy |
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Peter Dahlberg, Stanford University
Correlating Cryogenic Light and Electron Microscopy at the Nanoscale |
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Andres Jaschke, Heidelberg University
Short Talk: Illuminating RNA in Living Mammalian Cells: NIR Aptamer Advances for Super-Resolved Imaging |
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| 9:00–9:20 AM |
Coffee Break |
Longs Peak Foyer |
| 11:00–2:30 PM |
On Own for Lunch |
|
| 3:00–4:30 PM |
Symposia Spotlight 2: Technological Breakthroughs |
Longs Peak |
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Philipp S Erdmann, Human Technopole
SOLIST: Unlocking Molecular Landscapes Across Scales |
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Daphne-Eleni Archonta, Harvard University
VIS-À-VIS: Visualization and Vitrification after Stimulation |
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* Shawn Zheng, CZ Imaging Institute
High throughput Real-time Reconstruction of Fully Rorrected and Contrast Enhanced cryoET Tomograms |
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Léa Lecomte, EMBL - Grenoble
EasyGrid: A Versatile Platform for Automated Cryo-EM Sample Preparation and Control |
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Kexin Zhang, UMass Chan Medical School
Improved Cryo-EM Reconstruction of Sub-50 kDa Complexes using 2DTM |
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Lindsey Young, University of California, San Diego
Expanding the ToolKit for Cellular Tomography: Delivery into Live Cells of a cryo-CLEM Nanogold Tag for Structural Cell Biology |
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| 4:30–5:00 PM |
Coffee Available |
Longs Peak Foyer |
| 5:00–6:15 PM |
Bringing Molecules to Life with Live Cell Imaging at High Resolution |
Longs Peak |
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* Peter Dahlberg, Stanford University
Session Chair |
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Suliana Manley, École Polytechnique Fédérale de Lausanne
Mitochondrial Dynamic and Structural States Underpinning Homeostasis |
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Ralf Jungmann, Max Planck Institute of Biochemistry
DNA-PAINTs, SOMAmers and Ultra-Resolution Fluorescence Localizomics of DNA, RNA and Protein Interactions |
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Thomas A Hensel, Max Planck Institute for Multidisciplinary Natural Sciences
Short Talk: Co-Localization and Dynamics of Multiple Fluorescent Labels at the Molecular Scale |
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| 6:15–6:30 PM |
Meeting Wrap-Up: Outcomes and Future Directions (Organizers) |
Longs Peak |
| 7:00–8:00 PM |
Social Hour with Lite Bites |
Shavano/Torreys Peaks |
| 8:00–9:00 PM |
Entertainment |
Longs Peak |
| 8:00–9:00 PM |
Cash Bar |
Shavano/Torreys Peaks |
